H Md. Hafijur Rahman

About

Working at the bench and on the computer

I started in the wet lab — virology, vaccines, molecular biology — and moved toward computation without leaving the bench behind. The two inform each other.

I'm a Master's student in Biomedical Technology at Dokuz Eylül University, İzmir. My lab background is in virology, vaccine development and molecular biology; my computational work covers deep learning, immunopeptidomics, molecular modelling and multi-omics. My current research focuses on deep learning–based prediction of protein–protein interactions.

What I care about most is research that can be checked. My recent projects are preregistered where possible, log every decision, and keep withdrawn results on the record rather than quietly removing them.

What I do

At the bench

  • Virus propagation & attenuation in embryonated eggs
  • Vaccine formulation, animal trials & antibody analysis
  • PCR, DNA/RNA extraction & molecular biology
  • Microbial culture & antimicrobial assays
  • In vitro testing of plant extracts

On the computer

  • Deep learning for peptide & protein data
  • Immunopeptidomics & mass-spectrometry reanalysis
  • Molecular docking & dynamics simulation
  • Multi-omics, statistics & visualisation
  • Reproducible, preregistered pipelines

Bench toolkit

Molecular biology

  • PCR & PCR-product purification
  • DNA/RNA extraction
  • DNA isolation & quantification
  • Restriction digestion
  • Agarose gel electrophoresis

Microbiology & antimicrobial

  • Microbial cell culture
  • Morphological & biochemical assays
  • Cell counting
  • Antibiotic potency assay
  • Disc diffusion screening
  • Serial dilution (MIC)

Immunoassays

  • ELISA
  • Hepatitis-B detection (kit)

Virology & vaccines

  • Viral replication in chick embryo
  • Attenuation by serial passage
  • Vaccine formulation with adjuvant
  • Vaccine trials in chickens
  • Antibody analysis

Biochemistry

  • Carbohydrate, protein & sugar assays
  • Molisch, Benedict, Ninhydrin, Biuret, Nelson–Somogyi

Analytical & separation

  • Paper & column chromatography
  • Ion-exchange column (resin)
  • Water analysis: pH, turbidity, BOD, DO, membrane filtration

Computational toolkit

PythonPyTorchNumPy / SciPypandasscikit-learnMatplotlib ESM-2MHCflurryNetMHCpanBiopythonDocking & MD Jupyter / ColabDockerGit

Training

Leadership & volunteering

Languages

Bengali — nativeEnglish — C1Turkish — C1